| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Activator of 90 kDa heat shock protein ATPase homolog 1; AHA1; p38; AHSA1; C14orf3; HSPC322 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human AHA1 recombinant protein (Position: N21-F338). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AHSA1 (N-alpha-acetyltransferase 15, NatA auxiliary subunit) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-AHA1/AHSA1 Antibody Picoband® catalog # A05733. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human AHA1 recombinant protein (Position: N21-F338). (reported region: N21-F338).
- Molecular weight context: reported MW: 40 kDa; calculated MW: 101272 MW
- Reactivity: Human,Monkey
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
N-alpha-acetyltransferase 15, NatA auxiliary subunit; activator of HSP90 ATPase activity 1. Activator of 90 kDa heat shock protein ATPase homolog 1 is an enzyme that in humans is encoded by the AHSA1 gene. The International Radiation Hybrid Mapping Consortium mapped the AHSA1 gene to chromosome 14. It acts as a co-chaperone of HSP90AA1. AHSA1 competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins Functional note: Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity. Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Reported localization: Cytoplasm, cytosol. Expression/tissue context: Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
Research relevance and current trends
- Chaperones: Researchers commonly examine how AHSA1 (N-alpha-acetyltransferase 15, NatA auxiliary subunit) relates to this theme using model systems and orthogonal readouts.
- Golgi Proteins: Researchers commonly examine how AHSA1 (N-alpha-acetyltransferase 15, NatA auxiliary subunit) relates to this theme using model systems and orthogonal readouts.
- Protein Trafficking: Researchers commonly examine how AHSA1 (N-alpha-acetyltransferase 15, NatA auxiliary subunit) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AHSA1 (N-alpha-acetyltransferase 15, NatA auxiliary subunit) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
