| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aryl hydrocarbon receptor;Ah receptor;AhR;Class E basic helix-loop-helix protein 76;bHLHe76;AHR;BHLHE76; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human AhR |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-AhR Rabbit Monoclonal Antibody is an antibody targeting AHR. Common applications include WB, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOHI-1; isotype: Rabbit IgG; reactivity: Human; observed MW: 96,105 kDa; calculated MW: 96147 MW.
Boster Bio Anti-AhR Rabbit Monoclonal Antibody catalog # M00225. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: AHR — Aryl hydrocarbon receptor
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOHI-1; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 96,105 kDa; Calculated: 96147 MW
Biological background
Protein function (datasheet): Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1. .
Cellular localization (datasheet): Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
Tissue details (datasheet): Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. .
Research relevance and current trends
- Commonly studied in contexts related to Epigenetics and Nuclear Signaling,NFKB Pathway,Nuclear Signaling Pathways.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
