| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aryl hydrocarbon receptor;Ah receptor;AhR;Class E basic helix-loop-helix protein 76;bHLHe76;AHR;BHLHE76; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AhR |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOHI-1 is a rabbit monoclonal antibody that recognizes aryl hydrocarbon receptor (gene AHR) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 96.1 kDa; the supplier reports observed bands at 96,105 kDa.
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that senses environmental chemicals such as dioxins as well as dietary, microbial and metabolic compounds. After ligand binding it moves to the nucleus, pairs with ARNT and switches on genes such as CYP1A1, and it is widely studied in toxicology, immunity and cancer.
| Target | Aryl hydrocarbon receptor (gene AHR; UniProt P35869, human) |
|---|---|
| Clone | AOHI-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 96.1 kDa |
| Observed MW | 96,105 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human RT4 cells, human U-87MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ahR.
Reactivity Notes
The supplier lists reactivity with human. UniProt places aryl hydrocarbon receptor in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xi Yang et al. (2024) Aryl hydrocarbon receptor (AhR) is regulated by hyperoxia in premature infants. Journal of Maternal-Fetal & Neonatal Medicine. 10.1080/14767058.2024.2349179
