| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptosis-inducing factor 1, mitochondrial;1.1.1.-;Programmed cell death protein 8;AIFM1;AIF, PDCD8; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human AIF, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects apoptosis-inducing factor 1, mitochondrial (gene AIFM1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 66.9 kDa, and the supplier reports an observed band at about 67 kDa.
Apoptosis-inducing factor (AIF, AIFM1) is a flavoprotein of the mitochondrial intermembrane space with NADH oxidoreductase activity that supports respiratory chain function. After apoptotic stimuli it relocates to the nucleus, where it promotes caspase-independent DNA fragmentation, including in the cell death form called parthanatos.
| Target | Apoptosis-inducing factor 1, mitochondrial (gene AIFM1; UniProt O95831, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 66.9 kDa |
| Observed MW | 67 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human MCF-7 cells, human K562 cells, human A549 cells, human HepG2 cells, human HeLa cells, human Jurkat cells, human 293T cells, human U251 cells, rat stomach tissue, rat kidney tissue, rat NRK cells, mouse stomach tissue, mouse kidney tissue, mouse pancreas tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat cardiac muscle tissue, human intestinal cancer tissue, mouse cardiac muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): NIH3T3 cells, MCF-7 cells.
Samples with confirmed staining (ICC/IF): SMMC-7721 cells.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Synthetic peptide from the C-terminal region of human AIF. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places apoptosis-inducing factor 1, mitochondrial in the mitochondrion intermembrane space, mitochondrion inner membrane, cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in all tested tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Wang et al. (2023) Astaxanthin Protects against Alcoholic Liver Injury via Regulating Mitochondrial Redox Balance and Calcium Homeostasis. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.3c05529
Hong Zhang et al. (2017) RETRACTED: Icariin induces apoptosis in acute promyelocytic leukemia by targeting PIM1. Pharmacological Reports. 10.1016/j.pharep.2017.06.005
Yan Hou et al. (2008) Protection by bicyclol derivatives against acetaminophen-induced acute liver failure in mice and its active mechanism. Liver International. 10.1111/j.1478-3231.2008.01757.x
Feng Tianda et al. (2014) Protective Effects of Nigranoic Acid on Cerebral Ischemia–Reperfusion Injury and its Mechanism Involving Apoptotic Signaling Pathway. Cell Biochemistry and Biophysics. 10.1007/s12013-014-0204-1
