| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon-inducible protein AIM2; Interferon-inducible protein 210; Ifi-210; Interferon-inducible protein p210; Aim2; Gm1313; Ifi210 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Aim2 recombinant protein (Position: Q136-E354). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes interferon-inducible protein AIM2 in mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 40.2 kDa, and the supplier reports an observed band at about 39 kDa.
AIM2 (absent in melanoma 2) is an interferon-inducible sensor that detects double-stranded DNA in the cytosol. On binding DNA it assembles an inflammasome with ASC (PYCARD) and caspase-1, leading to IL-1β and IL-18 maturation and pyroptosis, so it is studied in antiviral and antibacterial immunity and autoinflammation.
| Target | Interferon-inducible protein AIM2 (gene Aim2; UniProt Q91VJ1, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.2 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse RAW264.7 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Recombinant mouse Aim2 fragment (Gln136–Glu354), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places interferon-inducible protein AIM2 in the cytoplasm, inflammasome and nucleus. Tissue expression noted by UniProt (mouse): Expressed in developing neurons. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
