| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | A-kinase anchor protein 14;AKAP-14;A-kinase anchor protein 28 kDa;AKAP 28;Protein kinase A-anchoring protein 14;PRKA14;AKAP14;AKAP28; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human AKAP14 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-AKAP14 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone FGO-1; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-AKAP14 Rabbit Monoclonal Antibody catalog # M15886. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: AKAP14 (A-kinase anchor protein 14).
- Antibody format: Monoclonal; clone FGO-1; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
AKAP14 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binds to type II regulatory subunits of protein kinase A and anchors/targets them. Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Widely expressed with highest levels in brain, kidney and muscle. .
Research relevance and current trends
- Research context keywords from the source record include: Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate AKAP14 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect AKAP14 expression by Western blot in cell or tissue lysates, Localize AKAP14 by immunofluorescence/immunocytochemistry in cultured cells, Enrich AKAP14 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 25 kDa; calculated MW: 22815 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 25 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Widely expressed with highest levels in brain, kidney and muscle. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
