| Field | Specification |
|---|---|
| Target | |
| Alternative names | A-kinase anchor protein 2;AKAP-2;AKAP-KL;Protein kinase A-anchoring protein 2;PRKA2;AKAP2;KIAA0920, PRKA2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human AKAP2, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes PALM2-AKAP2 fusion protein (gene PALM2AKAP2) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 94.7 kDa, and the supplier reports an observed band at about 150–170 kDa.
AKAP2 is an A-kinase anchoring protein that binds the RII regulatory subunit of protein kinase A. By tethering PKA to specific sites, it helps focus cAMP signaling, and it also associates with the actin cytoskeleton; the gene can be read through from the upstream PALM2 locus to give a PALM2–AKAP2 fusion protein.
| Target | PALM2-AKAP2 fusion protein (gene PALM2AKAP2; UniProt Q9Y2D5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 94.7 kDa |
| Observed MW | 150–170 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|
Samples with a confirmed band (WB): human PC-3 cells, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human AKAP2. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places PALM2-AKAP2 fusion protein in the apical cell membrane. Tissue expression noted by UniProt (human): Expressed in infantile heart and muscle, and fibroblasts. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
