| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aldo-keto reductase family 1 member B10;1.1.1.-;ARL-1;Aldose reductase-like;Aldose reductase-related protein;ARP;hARP;Small intestine reductase;SI reductase;AKR1B10;AKR1B11; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human AKR1B10. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes aldo-keto reductase family 1 member B10 (gene AKR1B10) in human samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 36.0 kDa, and the supplier reports an observed band at about 36 kDa.
AKR1B10 is an NADPH-dependent aldo-keto reductase with strong activity toward retinals and reactive carbonyls such as 4-hydroxynonenal and crotonaldehyde. It helps detoxify dietary and lipid-derived aldehydes, and its expression in epithelial tissues and tumors makes it a subject of research in retinoid metabolism and cancer.
| Target | Aldo-keto reductase family 1 member B10 (gene AKR1B10; UniProt O60218, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 36.0 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human A549 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of human AKR1B10.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes aldo-keto reductase family 1 member B10 as secreted and also located in the lysosome. Tissue expression noted by UniProt (human): Found in many tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yizhou Yao et al. (2020) Loss of AKR1B10 promotes colorectal cancer cells proliferation and migration via regulating FGF1-dependent pathway. Aging-US. 10.18632/aging.103393
