| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aldo-keto reductase family 1 member B10;1.1.1.-;ARL-1;Aldose reductase-like;Aldose reductase-related protein;ARP;hARP;Small intestine reductase;SI reductase;AKR1B10;AKR1B11; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human AKR1B10. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AKR1B10 (Aldo-keto reductase family 1 member B10) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-AKR1B10 Antibody Picoband® catalog # A02976. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human AKR1B10.
- Molecular weight context: reported MW: 36 kDa; calculated MW: 36020 MW
- Reactivity: Human
- Applications: IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Aldo-keto reductase family 1 member B10; Aldo-keto reductase family 1 member B10. Aldo-keto reductase family 1 member B10 is an enzyme that in humans is encoded by the AKR1B10 gene. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. Functional note: Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. . Reported localization: Lysosome . Secreted . Secreted through a lysosome-mediated non-classical pathway. Expression/tissue context: Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
Research relevance and current trends
- Cancer: Researchers commonly examine how AKR1B10 (Aldo-keto reductase family 1 member B10) relates to this theme using model systems and orthogonal readouts.
- Drug Metabolism: Researchers commonly examine how AKR1B10 (Aldo-keto reductase family 1 member B10) relates to this theme using model systems and orthogonal readouts.
- Energy Metabolism: Researchers commonly examine how AKR1B10 (Aldo-keto reductase family 1 member B10) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AKR1B10 (Aldo-keto reductase family 1 member B10) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of AKR1B10 (Aldo-keto reductase family 1 member B10) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
