| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aldo-keto reductase family 1 member C2;1.-.-.-;3-alpha-HSD3;Chlordecone reductase homolog HAKRD;Dihydrodiol dehydrogenase 2;DD-2;DD2;Dihydrodiol dehydrogenase/bile acid-binding protein;DD/BABP;Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase;1.3.1.20 ;Type III 3-alpha-hydroxysteroid dehydrogenase;1.1.1.357 ;AKR1C2;DDH2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E. coli-derived human AKR1C1/C2 recombinant protein (Position: M1-K123). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AKR1C2 (Aldo-keto reductase family 1 member C1/C2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-AKR1C1/C2 Antibody Picoband® catalog # PB10036. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human AKR1C1/C2 recombinant protein (Position: M1-K123). (reported region: M1-K123).
- Molecular weight context: reported MW: 37 kDa; calculated MW: 36735 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Aldo-keto reductase family 1 member C1/C2; Aldo-keto reductase family 1 member C2. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. And this gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. Functional note: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. . Reported localization: Cytoplasm . Expression/tissue context: Expressed in fetal testes. Expressed in fetal and adult adrenal glands. .
Research relevance and current trends
- Cancer: Researchers commonly examine how AKR1C2 (Aldo-keto reductase family 1 member C1/C2) relates to this theme using model systems and orthogonal readouts.
- Developmental Biology: Researchers commonly examine how AKR1C2 (Aldo-keto reductase family 1 member C1/C2) relates to this theme using model systems and orthogonal readouts.
- Drug Metabolism: Researchers commonly examine how AKR1C2 (Aldo-keto reductase family 1 member C1/C2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AKR1C2 (Aldo-keto reductase family 1 member C1/C2) levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
