| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RAC, PKB, PRKBA, AKT |
| Clonality | |
| Concentration | |
| Formulation | |
| Immunogen | The antibody was produced against synthesized non-phosphopeptide derived from human AKT1/AKT2/AKT3 around the phosphorylation site of tyrosine 315/316/312 (P-E-YPL- A). |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Storage |
Product Overview
Research Use OnlyThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
Validated Applications
| Immunohistochemistry (IHC) | 1:50~1:100 |
|---|
Key Specifications
| Clonality | Polyclonal |
|---|---|
| Species Reactivity | Human,Mouse,Rat |
| Isotype | IgG |
| Concentration | 100ug/100ul |
| Formulation | Liquid |
| Catalog No. | Y021501 |
| Alternative Names | RAC, PKB, PRKBA, AKT |
Immunogen / Antigen
The antibody was produced against synthesized non-phosphopeptide derived from human AKT1/AKT2/AKT3 around the phosphorylation site of tyrosine 315/316/312 (P-E-YPL- A).
Reactivity Notes
AKT1/AKT2/AKT3 (Ab-315/316/312) Antibody detects endogenous levels of total AKT1/AKT2/AKT3 protein.
Additional Information
Application Notes: IHC: 1:50~1:100
Storage & Handling
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Immunohistochemistry. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
IHC: 1:50~1:100
This antibody has been confirmed reactive against: Human,Mouse,Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
The antibody was produced against synthesized non-phosphopeptide derived from human AKT1/AKT2/AKT3 around the phosphorylation site of tyrosine 315/316/312 (P-E-YPL- A).
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
