| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase; AlaRS; Protein lactyltransferase AARS1; Renal carcinoma antigen NY-REN-42; AARS1; AARS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AlaRS/AARS1 recombinant protein (Position: R729-N968). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes alanine--tRNA ligase, cytoplasmic (gene AARS1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 106.8 kDa, and the supplier reports an observed band at about 107 kDa.
Alanyl-tRNA synthetase 1 (AARS1) charges tRNA(Ala) with alanine in the cytoplasm and uses a separate editing domain to remove wrongly attached amino acids, protecting the accuracy of protein synthesis. Mutations in AARS1 are linked to inherited neuropathies such as Charcot-Marie-Tooth disease.
| Target | Alanine--tRNA ligase, cytoplasmic (gene AARS1; UniProt P49588, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 106.8 kDa |
| Observed MW | 107 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human HepG2 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human AlaRS/AARS1 fragment (Arg729–Asn968), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places alanine--tRNA ligase, cytoplasmic in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
