| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5-aminolevulinate synthase, nonspecific, mitochondrial;ALAS-H;2.3.1.37;5-aminolevulinic acid synthase 1;Delta-ALA synthase 1;Delta-aminolevulinate synthase 1;ALAS1;ALAS3, ALASH;OK/SW-cl.121; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Alas1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EOA-1 is a rabbit monoclonal antibody that recognizes 5-aminolevulinate synthase, non-specific, mitochondrial (gene ALAS1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 70.6 kDa, and the supplier reports an observed band at about 71 kDa.
ALAS1 is the housekeeping, mitochondrial form of 5-aminolevulinate synthase. Using pyridoxal phosphate, it joins glycine and succinyl-CoA to make 5-aminolevulinic acid, the first and rate-limiting step of heme synthesis in non-erythroid tissues such as the liver, and it is studied in heme metabolism and the porphyrias.
| Target | 5-aminolevulinate synthase, non-specific, mitochondrial (gene ALAS1; UniProt P13196, human) |
|---|---|
| Clone | EOA-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 70.6 kDa |
| Observed MW | 71 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human alas1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places 5-aminolevulinate synthase, non-specific, mitochondrial in the mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
