| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 5-aminolevulinate synthase, nonspecific, mitochondrial;ALAS-H;2.3.1.37;5-aminolevulinic acid synthase 1;Delta-ALA synthase 1;Delta-aminolevulinate synthase 1;ALAS1;ALAS3, ALASH;OK/SW-cl.121; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Alas1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Alas1/Alas H Rabbit Monoclonal Antibody is an antibody targeting ALAS1. Common applications include WB, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: EOA-1; isotype: Rabbit IgG; reactivity: Human; observed MW: 10-12 kDa; calculated MW: 70581 MW.
Boster Bio Anti-Alas1/Alas H Rabbit Monoclonal Antibody catalog # M10405. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: ALAS1 — 5-aminolevulinate synthase, nonspecific, mitochondrial
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: EOA-1; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 10-12 kDa; Calculated: 70581 MW
Biological background
Protein function (datasheet): F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity). .
Cellular localization (datasheet): Mitochondrion matrix.
Tissue details (datasheet): Highly expressed in skeletal muscle, heart, liver and testis. .
Research relevance and current trends
- Commonly studied in contexts related to Cardiovascular,Metabolism,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
