| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alcohol dehydrogenase class-3;1.1.1.1;Alcohol dehydrogenase 5;Alcohol dehydrogenase class chi chain;Alcohol dehydrogenase class-III;Glutathione-dependent formaldehyde dehydrogenase;FALDH;FDH;GSH-FDH;1.1.1.-;S- (hydroxymethyl)glutathione dehydrogenase;1.1.1.284;ADH5 ;ADHX, FDH; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ADH5 recombinant protein (Position: K212-I374). Human ADH5 shares 90.2% and 92% amino acid (aa) sequence identity with mouse and rat ADH5, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes alcohol dehydrogenase class-3 (gene ADH5) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 39.7 kDa, and the supplier reports an observed band at about 39 kDa.
Alcohol dehydrogenase class-3 (ADH5) is better known as S-nitrosoglutathione reductase (GSNOR). It detoxifies formaldehyde through a glutathione adduct and breaks down S-nitrosoglutathione, so it regulates protein S-nitrosylation and is studied in nitric oxide signaling.
| Target | Alcohol dehydrogenase class-3 (gene ADH5; UniProt P11766, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 39.7 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human K562 cells, human MOLT4 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Recombinant human ADH5 fragment (Lys212–Ile374), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 90.2% and 92%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alcohol dehydrogenase class-3 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
