| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alcohol dehydrogenase class-3;1.1.1.1;Alcohol dehydrogenase 5;Alcohol dehydrogenase class chi chain;Alcohol dehydrogenase class-III;Glutathione-dependent formaldehyde dehydrogenase;FALDH;FDH;GSH-FDH;1.1.1.-;S- (hydroxymethyl)glutathione dehydrogenase;1.1.1.284;ADH5 ;ADHX, FDH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human ADH5 recombinant protein (Position: K212-I374). Human ADH5 shares 90.2% and 92% amino acid (aa) sequence identity with mouse and rat ADH5, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ADH5 (Alcohol dehydrogenase class-3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Alcohol dehydrogenase class-3 ADH5 Antibody catalog # RP1111. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human ADH5 recombinant protein (Position: K212-I374). Human ADH5 shares 90.2% and 92% amino acid (aa) sequence identity with mouse and rat ADH5, respectively. (reported region: K212-I374).
- Molecular weight context: reported MW: 39 kDa; calculated MW: 39724 MW
- Reactivity: Human,Mouse,Rat
- Applications: IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-3. Alcohol dehydrogenase class-3 is an enzyme that in humans is encoded by the ADH5 gene. This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. Functional note: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S- (hydroxymethyl) glutathione. Reported localization: Cytoplasm. Expression/tissue context: Detected in cardiac myocytes.
Research relevance and current trends
- Metabolic Signaling Pathways: Researchers commonly examine how ADH5 (Alcohol dehydrogenase class-3) relates to this theme using model systems and orthogonal readouts.
- Metabolism: Researchers commonly examine how ADH5 (Alcohol dehydrogenase class-3) relates to this theme using model systems and orthogonal readouts.
- Pathways and Processes: Researchers commonly examine how ADH5 (Alcohol dehydrogenase class-3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ADH5 (Alcohol dehydrogenase class-3) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ADH5 (Alcohol dehydrogenase class-3) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
