| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death 6-interacting protein;PDCD6-interacting protein;ALG-2-interacting protein 1;ALG-2-interacting protein X;Hp95;PDCD6IP;AIP1, ALIX, KIAA1375; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ALIX recombinant protein (Position: A2-D330). Human ALIX shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat ALIX, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes programmed cell death 6-interacting protein (gene PDCD6IP) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 96.0 kDa, and the supplier reports an observed band at about 96 kDa.
ALIX (PDCD6IP) is a multifunctional adaptor of the ESCRT machinery. It links cargo sorting at endosomes to ESCRT-III proteins such as CHMP4, supporting formation of intralumenal vesicles in multivesicular bodies, exosome release and the final abscission step of cytokinesis. Because of these roles, ALIX is a widely used exosome marker and is studied in membrane repair, virus budding and apoptosis.
| Target | Programmed cell death 6-interacting protein (gene PDCD6IP; UniProt Q8WUM4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 96.0 kDa |
| Observed MW | 96 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, rat liver tissue, rat RH35 cells, mouse liver tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with confirmed staining (ICC/IF): human pancreatic cancer tissue, human prostate cancer tissue.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human ALIX fragment (Ala2–Asp330), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.7% and 95.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes programmed cell death 6-interacting protein as secreted and also located in the cytoplasm, melanosome and cell junction. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yumei Li et al. (2020) Galectin-3 mediates pulmonary vascular endothelial cell dynamics via TRPC1/4 under acute hypoxia. Journal of Biochemical and Molecular Toxicology. 10.1002/jbt.22463
Li Ling et al. (2019) CircRNAs in exosomes from high glucose-treated glomerular endothelial cells activate mesangial cells. American Journal of Translational Research. PMID: 31497190
Cao Guijun et al. (2020) Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a. Bioscience Reports. 10.1042/BSR20193436
Lingjuan Du et al. (2018) Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A. International Journal of Clinical and Experimental Pathology. PMID: 31949563
