| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA demethylase ALKBH5; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; ALKBH5; ABH5; OFOXD1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ALKBH5 recombinant protein (Position: E81-R180). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes RNA demethylase ALKBH5 in human samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 44.3 kDa, and the supplier reports an observed band at about 52 kDa.
ALKBH5 is an Fe(II)/α-ketoglutarate-dependent dioxygenase that removes N6-methyladenosine (m6A) from RNA, acting as an m6A "eraser". By changing m6A levels, it affects mRNA processing, export and translation, and it is required for normal spermatogenesis and studied in cancer.
| Target | RNA demethylase ALKBH5 (gene ALKBH5; UniProt Q6P6C2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 44.3 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human Jurkat cells, human HeLa cells, human HepG2 cells, human U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Recombinant human ALKBH5 fragment (Glu81–Arg180), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places RNA demethylase ALKBH5 in the nucleus speckle and nucleus. Tissue expression noted by UniProt (human): Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Fengkai Ruan et al. (2021) RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological Assessment. Small Methods. 10.1002/smtd.202001045
Fengkai Ruan et al. (2022) Role of RNA m6A modification in titanium dioxide nanoparticle-induced acute pulmonary injury: An in vitro and in vivo study. Environmental Pollution. 10.1016/j.envpol.2022.119986
Shen Wenyi et al. (2022) The RNA demethylase ALKBH5 promotes the progression and angiogenesis of lung cancer by regulating the stability of the LncRNA PVT1. Cancer Cell International. 10.1186/s12935-022-02770-0
Zhuang Yong et al. (2025) ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma. Scientific Reports. 10.1038/s41598-024-84352-w
