| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Sodium/potassium-transporting ATPase subunit alpha-1;Na (+)/K (+) ATPase alpha-1 subunit;3.6.3.9;Sodium pump subunit alpha-1;ATP1A1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ATP1A1, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ATP1A1 (Sodium/potassium-transporting ATPase subunit alpha-1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband® catalog # PB9482. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human ATP1A1, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
- Molecular weight context: reported MW: 100-113 kDa; calculated MW: 112896 MW
- Reactivity: Human,Mouse,Rat
- Applications: IF, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Sodium/potassium-transporting ATPase subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1. The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. Functional note: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Reported localization: Cell membrane; Multi-pass membrane protein. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Expression/tissue context: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .
Research relevance and current trends
- ATPases: Researchers commonly examine how ATP1A1 (Sodium/potassium-transporting ATPase subunit alpha-1) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how ATP1A1 (Sodium/potassium-transporting ATPase subunit alpha-1) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how ATP1A1 (Sodium/potassium-transporting ATPase subunit alpha-1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ATP1A1 (Sodium/potassium-transporting ATPase subunit alpha-1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ATP1A1 (Sodium/potassium-transporting ATPase subunit alpha-1) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the ATG14 family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
