| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Calnexin;IP90;Major histocompatibility complex class I antigen-binding protein p88;p90;CANX; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human alpha 1 Spectrin |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-SPTA1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23S53; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-alpha 1 Spectrin Rabbit Monoclonal Antibody catalog # M03376-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: SPTA1 (Calnexin).
- Antibody format: Monoclonal; clone 23S53; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
SPTA1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor- mediated endocytosis at the synapse. Reported cellular localization context: Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endoplasmic reticulum . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232). . Tissue expression notes (as provided): Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine. .
Research relevance and current trends
- Research context keywords from the source record include: Actin Binding Proteins,Actin, etc.,Cardiovascular,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate SPTA1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect SPTA1 expression by Western blot in cell or tissue lysates, Localize SPTA1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify SPTA1-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 280 kDa; calculated MW: 67568 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 280 kDa
- Cellular localization (provided): Endoplasmic reticulum membrane ; Single-pass type I membrane protein . Endoplasmic reticulum . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232). .
- Tissue details (provided): Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
