| Field | Specification |
|---|---|
| Target | |
| Alternative names | Spectrin alpha chain, erythrocytic 1; Erythroid alpha-spectrin; SPTA1; SPTA |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human alpha 1 Spectrin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23S53 is a rabbit monoclonal antibody that recognizes spectrin alpha chain, erythrocytic 1 (gene SPTA1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 280.0 kDa, and the supplier reports an observed band at about 280 kDa.
Spectrin alpha chain, erythrocytic 1 (SPTA1) pairs with β-spectrin to form the flexible network that lines the red blood cell membrane. Together with actin and protein 4.1, this skeleton gives erythrocytes their shape and deformability, and SPTA1 mutations cause hereditary spherocytosis and elliptocytosis.
| Target | Spectrin alpha chain, erythrocytic 1 (gene SPTA1; UniProt P02549, human) |
|---|---|
| Clone | 23S53 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 280.0 kDa |
| Observed MW | 280 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human alpha 1 Spectrin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places spectrin alpha chain, erythrocytic 1 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tudor Sandulescu et al. (2020) Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system. Annals of Anatomy-Anatomischer Anzeiger. 10.1016/j.aanat.2020.151647
