| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Adaptor protein complex AP-2, alpha 1 subunit (Predicted) ;Protein Ap2a1 ;Ap2a1 ;Ap2a1_predicted ;rCG_54084 ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | AP-2 adaptor polypeptides from bovine brain. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) is an antibody targeting AP2A1. Common applications include WB. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: 100/2; isotype: Mouse IgG2a; reactivity: Human,Mouse,Rat; observed MW: 108 kDa; calculated MW: 107675 MW.
Boster Bio Anti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) catalog # MA1105. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: AP2A1 — adaptor-related protein complex 2, alpha 1 subunit
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: 100/2; Isotype: Mouse IgG2a
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 108 kDa; Calculated: 107675 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): AP2A1: Component of the adaptor protein complex 2 (AP-2).Adaptor protein complexes function in protein transport viatransport vesicles in different membrane traffic pathways. Adaptorprotein complexes are vesicle coat components and appear to beinvolved in cargo selection and vesicle formation. AP-2 isinvolved in clathrin-dependent endocytosis in which cargo proteinsare incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with theearly endosome. The clathrin lattice serves as a mechanicalscaffold but is itself unable to bind ly to membranecomponents. Clathrin-associated adaptor protein (AP) complexeswhich can bind ly to both the clathrin lattice and to thelipid and protein components of membranes are considered to be themajor clathrin adaptors contributing the CCV formation. AP-2 alsoserves as a cargo receptor to selectively sort the membraneproteins involved in receptor-mediated endocytosis. AP-2 seems toplay a role in the recycling of synaptic vesicle membranes fromthe presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi)and [ED]-X-X-X-L-[LI] endocytosis signal motifs within thecytosolic tails of transmembrane cargo molecules. AP-2 may alsoplay a role in maintaining normal post-endocytic traffickingthrough the ARF6-regulated, non-clathrin pathway. The AP-2 alphasubunit binds polyphosphoinositide-containing lipids, positioningAP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocyticaccessory proteins. The AP-2 alpha and AP-2 sigma subunits arethought to contribute to the recognition of the [ED]-X-X-X-L-[LI]motif (By similarity).
Scientific background (datasheet): Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
Cellular localization (datasheet): AP2A1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.|Ap2a1: Cell membrane Membrane,coated pit Note: AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV.
Tissue details (datasheet): AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells.
Sequence similarities (datasheet): Belongs to the adaptor complexes large subunit family.
Research relevance and current trends
- Commonly studied in contexts related to Adapters,Signal Transduction,Transmembrane.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
