| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha-sarcoglycan;Alpha-SG;50 kDa dystrophin-associated glycoprotein;50DAG;Adhalin;Dystroglycan-2;SGCA;ADL, DAG2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human SGCA recombinant protein (Position: Q24-H387). Human SGCA shares 90.7% amino acid (aa) sequence identity with mouse SGCA. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SGCA (Alpha-sarcoglycan) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-alpha Sarcoglycan/SGCA Antibody Picoband® catalog # PB9503. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human SGCA recombinant protein (Position: Q24-H387). Human SGCA shares 90.7% amino acid (aa) sequence identity with mouse SGCA. (reported region: Q24-H387).
- Molecular weight context: reported MW: 43 kDa; calculated MW: 42875 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Alpha-sarcoglycan; Alpha-sarcoglycan. Alpha-sarcoglycan is a protein that in humans is encoded by the SGCA gene. This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. Functional note: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Reported localization: Cell membrane, sarcolemma ; Single-pass type I membrane protein . Cytoplasm, cytoskeleton . Expression/tissue context: Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
Research relevance and current trends
- Cytoskeleton/ECM: Researchers commonly examine how SGCA (Alpha-sarcoglycan) relates to this theme using model systems and orthogonal readouts.
- ECM Proteins: Researchers commonly examine how SGCA (Alpha-sarcoglycan) relates to this theme using model systems and orthogonal readouts.
- Extracellular Matrix: Researchers commonly examine how SGCA (Alpha-sarcoglycan) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SGCA (Alpha-sarcoglycan) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the sarcoglycan alpha/epsilon family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
