| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Actin, aortic smooth muscle;Alpha-actin-2;Acta2;Actsa, Actvs; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | N-terminal synthetic decapeptide of alpha-smooth muscle actin. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) is an antibody targeting ACTN. Common applications include WB, IHC, IF, IHC-F. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: 1A4; isotype: Mouse IgG2a; reactivity: Human,Mouse,Rat; observed MW: 42 kDa; calculated MW: 42009 MW.
Boster Bio Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) catalog # MA1106. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ACTN — Actin, aortic smooth muscle
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: 1A4; Isotype: Mouse IgG2a
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 42 kDa; Calculated: 42009 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Scientific background (datasheet): Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
Cellular localization (datasheet): Cytoplasm, cytoskeleton.
Tissue details (datasheet): AP2A1: Isoform A expressed in forebrain, skeletalmuscle, spinal cord, cerebellum, salivary gland, heart and colon.Isoform B is widely expressed in tissues and also in breast cancerand in prostate carcinoma cells.
Sequence similarities (datasheet): Belongs to the adaptor complexes large subunit family.
Research relevance and current trends
- Commonly studied in contexts related to Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
