| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Angiotensinogen;Serpin A8;Angiotensin-1;Angiotensin 1-10;Angiotensin I;Ang I;Angiotensin-2;Angiotensin 1-8;Angiotensin II;Ang II;Angiotensin-3;Angiotensin 2-8;Angiotensin III;Ang III;Des-Asp[1]-angiotensin II;Angiotensin-4;Angiotensin 3-8;Angiotensin IV;Ang IV;Angiotensin 1-9;Angiotensin 1-7;Angiotensin 1-5;Angiotensin 1-4;Agt;Serpina8; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Angiotensinogen/AGT recombinant protein (Position: D34-R458). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Angiotensinogen/AGT Antibody Picoband® is an antibody for AGT detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: AGT (Angiotensinogen); UniProt: P01019
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 55 kDa, calculated 51990 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Angiotensinogen/AGT Antibody Picoband® catalog # A02103-3.
Biological background
Biological context: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
Expression and localization notes: cellular localization: Secreted., tissue context: Expressed by the liver and secreted in plasma..
Common research applications
- Western blotting (WB): Compare AGT levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of AGT in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify AGT-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: AGT(ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Secreted.
- Tissue details: Expressed by the liver and secreted in plasma.
- Research category: Cardiovascular,G Protein Signaling,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
