| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Annexin A10;Annexin-10;Annexin-14;ANXA10;ANX14; |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin A10, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Annexin A10/ANXA10 Antibody Picoband® is an antibody targeting ANXA10. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Rat,Human; observed MW: 50 kDa; calculated MW: 37278 MW.
Boster Bio Anti-Annexin A10/ANXA10 Antibody catalog # PA2077. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: ANXA10 — Annexin A10
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Rat,Human
- Molecular weight guidance: Observed: 50 kDa; Calculated: 37278 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Scientific background (datasheet): ANXA10 (Annexin A10) is a member of the annexin family of calcium-dependent phospholipid-binding proteins. By FISH, Morgan et al. (1999) mapped the ANXA10 gene to chromosome 4q33. Morgan et al. (1999) suggested that the ablation or depletion of principal calcium-binding sites in annexins A9 and A10, respectively, may dispose them to function in distinct tissue environments where calcium sensitivity is not a major functional determinant.
Sequence similarities (datasheet): Belongs to the annexin family.
Research relevance and current trends
- Commonly studied in contexts related to Calcium Binding Proteins,Calcium Signaling,Cell Adhesion,Cytoskeleton/ECM,Signal Transduction,Signaling Pathway.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
