| Field | Specification |
|---|---|
| Target | |
| Alternative names | Anterior gradient protein 2 homolog;AG-2;hAG-2;HPC8;Secreted cement gland protein XAG-2 homolog;AGR2;AG2;UNQ515/PRO1030; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Anterior Gradient 2 recombinant protein (Position: R21-L175). Human Anterior Gradient 2 shares 93.5% amino acid (aa) sequence identity with mouse Anterior Gradient 2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets anterior gradient protein 2 homolog (gene AGR2) in human, mouse and rat samples and is validated for flow cytometry, immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 20.0 kDa, and the supplier reports an observed band at about 17 kDa.
Anterior gradient 2 (AGR2) is a protein disulfide isomerase family member located in the endoplasmic reticulum. It is needed for proper production and secretion of the mucin MUC2 by intestinal cells, and its frequent overexpression in adenocarcinomas has made it a subject of research on cell migration, growth and tumor biology.
| Target | Anterior gradient protein 2 homolog (gene AGR2; UniProt O95994, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 20.0 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human T47D cells, human A549 cells, rat stomach tissue, rat small intestine tissue, mouse stomach tissue, mouse small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human non-small cells lung cancer tissue, human bladder infiltrating urothelial carcinoma with squamous differentiation tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed result (IP): A549 cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human Anterior Gradient 2 fragment (Arg21–Leu175), expressed in E. coli. Sequence identity with the mouse ortholog: 93.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes anterior gradient protein 2 homolog as secreted and also located in the endoplasmic reticulum. Tissue expression noted by UniProt (human): Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ying Zhu et al. (2025) Momordica Charantia L. polysaccharides ameliorate colonic mucus barrier damage induced by high fat diet through regulation of gut microbiota and MUC2 expression. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.148065
Manchun Huang et al. (2025) Arabinoxylan from barley bran alleviates ulcerative colitis in mice through enhancement of mucosal barrier function and modulation of gut microbiota. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.144941
