| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Anterior gradient protein 2 homolog;AG-2;hAG-2;HPC8;Secreted cement gland protein XAG-2 homolog;AGR2;AG2;UNQ515/PRO1030; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Anterior Gradient 2 recombinant protein (Position: R21-L175). Human Anterior Gradient 2 shares 93.5% amino acid (aa) sequence identity with mouse Anterior Gradient 2. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AGR2 (Anterior gradient protein 2 homolog) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Anterior Gradient 2/AGR2 Antibody Picoband® catalog # A02922-2. Tested in Flow Cytometry, IP, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Anterior Gradient 2 recombinant protein (Position: R21-L175). Human Anterior Gradient 2 shares 93.5% amino acid (aa) sequence identity with mouse Anterior Gradient 2. (reported region: R21-L175).
- Molecular weight context: reported MW: 20 kDa; calculated MW: 19979 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IP, ICC/IF, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Anterior gradient protein 2 homolog; Anterior gradient protein 2 homolog. Anterior gradient protein 2 homolog (AGR-2), also known as secreted cement gland protein XAG-2 homolog, is a protein that in humans is encoded by the AGR2 gene. Anterior gradient homolog 2 was originally discovered in Xenopus laevis. In Xenopus AGR2 plays a role in cement gland differentiation, but in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response, cell migration, and cell transformation. However, there have been other observations that AGR2 can repress growth and proliferation. Functional note: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. . Reported localization: Secreted . Endoplasmic reticulum . Expression/tissue context: Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. .
Research relevance and current trends
- Cancer: Researchers commonly examine how AGR2 (Anterior gradient protein 2 homolog) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how AGR2 (Anterior gradient protein 2 homolog) relates to this theme using model systems and orthogonal readouts.
- ECM Proteins: Researchers commonly examine how AGR2 (Anterior gradient protein 2 homolog) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AGR2 (Anterior gradient protein 2 homolog) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of AGR2 (Anterior gradient protein 2 homolog) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
