| Field | Specification |
|---|---|
| Target | |
| Alternative names | AP-2 complex subunit beta;AP105B;Adaptor protein complex AP-2 subunit beta;Adaptor-related protein complex 2 subunit beta;Beta-2-adaptin;Beta-adaptin;Clathrin assembly protein complex 2 beta large chain;Plasma membrane adaptor HA2/AP2 adaptin beta subunit;AP2B1;ADTB2, CLAPB1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human AP2B1 recombinant protein (Position: Q311-R522). Human AP2B1 shares 100% amino acid (aa) sequence identity with both mouse and rat AP2B1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects AP-2 complex subunit beta (gene AP2B1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 104.6 kDa, and the supplier reports an observed band at about 105 kDa.
AP2B1 is the β2 subunit of adaptor protein complex 2 (AP-2), the main clathrin adaptor at the plasma membrane. It binds clathrin directly and helps assemble clathrin-coated pits that internalize receptors and other cargo by endocytosis.
| Target | AP-2 complex subunit beta (gene AP2B1; UniProt P63010, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 104.6 kDa |
| Observed MW | 105 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human A549 cells, human K562 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human AP2B1 fragment (Gln311–Arg522), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 100%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places AP-2 complex subunit beta in the cell membrane and membrane. Tissue expression noted by UniProt (human): Expressed in the brain (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
