| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Adenomatous polyposis coli protein 2;Adenomatous polyposis coli protein-like;APC-like;APC2;APCL; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human APC2, different from the related mouse sequence by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of APC2 (Adenomatous polyposis coli protein 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-APC2 Antibody Picoband® catalog # PB10041. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human APC2, different from the related mouse sequence by two amino acids.
- Molecular weight context: reported MW: 94 kDa; calculated MW: 243949 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Adenomatous polyposis coli protein 2; Adenomatous polyposis coli protein 2. APC2, which is also called APCL, is a deduced 2,303-amino acid protein that contains an N-terminal coiled-coil domain, followed by an armadillo domain and five 20-amino acid repeats. The human APC2 gene is mapped to chromosome 19p13.3. It is found that the 20-amino acid repeat domain of APCL could bind beta-catenin (CTNNB1) and deplete the intracellular beta-catenin pool. A reporter gene assay revealed that APCL could regulate interaction of beta-catenin with T cell-specific transcription factors (TCF7), although less efficiently than APC. Functional note: Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling. . Reported localization: Cytoplasm, cytoskeleton . Golgi apparatus . Cytoplasm . Cytoplasm, perinuclear region . Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822). . Expression/tissue context: Widely expressed (at protein level). Specifically expressed in the CNS. .
Research relevance and current trends
- Cell Cycle: Researchers commonly examine how APC2 (Adenomatous polyposis coli protein 2) relates to this theme using model systems and orthogonal readouts.
- Cell Division: Researchers commonly examine how APC2 (Adenomatous polyposis coli protein 2) relates to this theme using model systems and orthogonal readouts.
- Proteasome / Ubiquitin: Researchers commonly examine how APC2 (Adenomatous polyposis coli protein 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative APC2 (Adenomatous polyposis coli protein 2) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
