Anti-Apg7/ATG7 Antibody Picoband®

Overview

This antibody is intended for detection of ATG7 (Ubiquitin-like modifier-activating enzyme ATG7) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-Apg7/ATG7 Antibody Picoband® catalog # PB9479. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7, different from the related mouse and rat sequences by two amino acids.
• Molecular weight context: reported MW: 78 kDa; calculated MW: 77960 MW
• Reactivity: Human,Mouse,Rat
• Applications: WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

Ubiquitin-like modifier-activating enzyme ATG7; Ubiquitin-like modifier-activating enzyme ATG7. Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways. Functional note: E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. . Reported localization: Cytoplasm . Preautophagosomal structure . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. . Expression/tissue context: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Research relevance and current trends

• Apoptosis: Researchers commonly examine how ATG7 (Ubiquitin-like modifier-activating enzyme ATG7) relates to this theme using model systems and orthogonal readouts.
• Associated Proteins: Researchers commonly examine how ATG7 (Ubiquitin-like modifier-activating enzyme ATG7) relates to this theme using model systems and orthogonal readouts.
• Cancer: Researchers commonly examine how ATG7 (Ubiquitin-like modifier-activating enzyme ATG7) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative ATG7 (Ubiquitin-like modifier-activating enzyme ATG7) levels across conditions; band patterns may reflect isoforms and processing.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins
• Family / similarity context: Belongs to the aspartyl/asparaginyl beta-hydroxylase family.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.