| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA dC->dU-editing enzyme APOBEC-3G;3.5.4.-;APOBEC-related cytidine deaminase;APOBEC-related protein;ARCD;APOBEC-related protein 9;ARP-9;CEM-15;CEM15;Deoxycytidine deaminase;A3G;APOBEC3G;MDS019; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human APOBEC3G recombinant protein (Position: E191-N384). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA dC->dU-editing enzyme APOBEC-3G (gene APOBEC3G) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 46.4 kDa, and the supplier reports an observed band at about 46 kDa.
APOBEC3G is a cytidine deaminase best known as an antiviral restriction factor against HIV-1. It is packaged into budding virions and deaminates cytosines in viral minus-strand DNA during reverse transcription, causing G-to-A hypermutation; the HIV-1 protein Vif counters it by targeting it for degradation, making APOBEC3G a key subject in HIV research.
| Target | DNA dC->dU-editing enzyme APOBEC-3G (gene APOBEC3G; UniProt Q9HC16, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.4 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human Jurkat cells lysate, human HEL cells lysate, human RT4 cells lysate, rat testis tissue lysates, rat PC-12 cells lysate, mouse testis tissue lysates, mouse RAW264.7 cells lysate.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Recombinant human APOBEC3G fragment (Glu191–Asn384), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA dC->dU-editing enzyme APOBEC-3G in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
