| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein A-I;Apo-AI;ApoA-I;Apolipoprotein A1;Proapolipoprotein A-I;ProapoA-I;Truncated apolipoprotein A-I;Apoa1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse APOA1 recombinant protein (Position: D25-Q264). Mouse APOA1 shares 64% and 68.6% amino acid (aa) sequence identity with human and rat APOA1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects apolipoprotein A-I (gene Apoa1) in mouse samples and is validated for IHC and Western blot. The predicted molecular weight is 30.6 kDa, and the supplier reports an observed band at about 25 kDa.
Apolipoprotein A-I (APOA1) is the main protein component of high-density lipoprotein (HDL). It helps remove excess cholesterol from peripheral tissues and carry it to the liver, and it activates lecithin-cholesterol acyltransferase (LCAT), which esterifies cholesterol within HDL particles. APOA1 is widely measured in studies of lipid metabolism and cardiovascular health.
| Target | Apolipoprotein A-I (gene Apoa1; UniProt Q00623, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 30.6 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse) |
Samples with a confirmed band (WB): mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, mouse kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse APOA1 fragment (Asp25–Gln264), expressed in E. coli. Sequence identity with the human and rat orthologs: 64% and 68.6%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes apolipoprotein A-I as a secreted protein. Tissue expression noted by UniProt (mouse): Major protein of plasma HDL, also found in chylomicrons. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Dongqi Liu et al. (2021) Rapid glycosylation analysis of mouse serum glycoproteins separated by supported molecular matrix electrophoresis. Journal of Proteomics. 10.1016/j.jprot.2020.104098
