| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein M; Apo-M; ApoM; Protein G3a; G3A; NG20 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ApoM |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23A72 is a rabbit monoclonal antibody that recognizes apolipoprotein M (gene APOM) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 21.3 kDa, and the supplier reports an observed band at about 25 kDa.
Apolipoprotein M (APOM) is a lipocalin-family apolipoprotein carried mainly on HDL. Its binding pocket carries sphingosine-1-phosphate (S1P) in plasma, and it can also bind retinoids and fatty acids, so it is studied in HDL function, S1P signaling and vascular biology.
| Target | Apolipoprotein M (gene APOM; UniProt O95445, human) |
|---|---|
| Clone | 23A72 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.3 kDa |
| Observed MW | 25 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:60 |
Samples with a confirmed band (WB): human plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human apoM.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes apolipoprotein M as a secreted protein. Tissue expression noted by UniProt (human): Plasma protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.