| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aquaporin-4;AQP-4;Mercurial-insensitive water channel;MIWC;WCH4;AQP4; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of AQP4 (Aquaporin-4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Aquaporin 4/AQP4 Antibody Picoband® catalog # PB9475. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
- Molecular weight context: reported MW: 37 kDa; calculated MW: 34830 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Aquaporin-4; Aquaporin-4. Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier. Functional note: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. Reported localization: Membrane; Multi-pass membrane protein. Expression/tissue context: Brain - muscle >> heart, kidney, lung, and trachea.
Research relevance and current trends
- Cancer: Researchers commonly examine how AQP4 (Aquaporin-4) relates to this theme using model systems and orthogonal readouts.
- Channels: Researchers commonly examine how AQP4 (Aquaporin-4) relates to this theme using model systems and orthogonal readouts.
- Metabolism: Researchers commonly examine how AQP4 (Aquaporin-4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative AQP4 (Aquaporin-4) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of AQP4 (Aquaporin-4) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the MIP/aquaporin (TC 1.A.8) family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
