| Field | Specification |
|---|---|
| Target | |
| Alternative names | ADP-ribosylation factor 6;ARF6; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ARF6 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOFF-1 is a rabbit monoclonal antibody that recognizes ADP-ribosylation factor 6 (gene ARF6) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 20.1 kDa, and the supplier reports an observed band at about 20 kDa.
ADP-ribosylation factor 6 (ARF6) is a small GTPase that acts at the plasma membrane and endosomes. It regulates endocytic recycling, phosphoinositide signaling and actin remodeling, and it is studied in cell migration, cell spreading, cytokinesis and tumor cell invasion.
| Target | ADP-ribosylation factor 6 (gene ARF6; UniProt P62330, human) |
|---|---|
| Clone | AOFF-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.1 kDa |
| Observed MW | 20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ARF6.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ADP-ribosylation factor 6 in the cytoplasm, cell membrane, endosome membrane and recycling endosome membrane. Tissue expression noted by UniProt (human): Ubiquitous, with higher levels in heart, substantia nigra, and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
