Anti-ARFGAP1 Antibody Picoband®

Overview

This antibody is intended for detection of ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-ARFGAP1 Antibody Picoband® catalog # A04959. Tested in Flow Cytometry, ICC, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Rabbit Polyclonal Rabbit IgG
• Immunogen / epitope context: E. coli-derived human ARFGAP1 recombinant protein (Position: M1-Y183). Human ARFGAP1 shares 89.1% and 88.5% amino acid (aa) sequence identity with mouse and rat ARFGAP1, respectively. (reported region: M1-Y183).
• Molecular weight context: reported MW: 45 kDa; calculated MW: 44668 MW
• Reactivity: Human,Mouse,Rat
• Applications: Flow Cytometry, ICC, IHC, IF, WB

As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.

Biological background

ADP-ribosylation factor GTPase-activating protein 1; ADP-ribosylation factor GTPase-activating protein 1. ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene. And this gene is mapped to 20q13.33. The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. Functional note: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity). . Reported localization: Cytoplasm . Golgi apparatus . Associates with the Golgi complex. . Expression/tissue context: Ubiquitous. .

Research relevance and current trends

• ER Proteins: Researchers commonly examine how ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) relates to this theme using model systems and orthogonal readouts.
• Golgi Proteins: Researchers commonly examine how ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) relates to this theme using model systems and orthogonal readouts.
• Protein Trafficking: Researchers commonly examine how ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) across tissue regions and cell types using matched controls.
• IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
• Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.