| Field | Specification |
|---|---|
| Target | |
| Alternative names | AT-rich interactive domain-containing protein 2; ARID domain-containing protein 2; BRG1-associated factor 200; BAF200; Zinc finger protein with activation potential; Zipzap/p200; ARID2; BAF200; KIAA1557 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ARID2, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects AT-rich interactive domain-containing protein 2 (gene ARID2) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 197.4 kDa, and the supplier reports an observed band at about 245 kDa.
ARID2 is a subunit specific to the PBAF form of the SWI/SNF chromatin remodeling complex. It helps the complex alter nucleosome positioning to control gene expression, and inactivating ARID2 mutations are found in several cancers, making it a subject of chromatin and cancer research.
| Target | AT-rich interactive domain-containing protein 2 (gene ARID2; UniProt Q68CP9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 197.4 kDa |
| Observed MW | 245 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| Immunoprecipitation | 0.5–2 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells, human Jurkat cells, human HEL cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed result (IP): MCF-7 cells.
Immunogen
Synthetic peptide from the N-terminal region of human ARID2. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human. UniProt places AT-rich interactive domain-containing protein 2 in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Amy E. Baxter et al. (2023) The SWI/SNF chromatin remodeling complexes BAF and PBAF differentially regulate epigenetic transitions in exhausted CD8+ T cells. Immunity. 10.1016/j.immuni.2023.05.008
Yang Jinwei et al. (2023) Comprehensive genomic profiling reveals prognostic signatures and insights into the molecular landscape of colorectal cancer. Frontiers in Oncology. 10.3389/fonc.2023.1285508
