| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Aromatase; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase; CYP19A1; ARO1; CYAR; CYP19 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CYP19A1 recombinant protein (Position: Y241-H503). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CYP19A1 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Aromatase/CYP19A1 Antibody Picoband® catalog # A00071. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CYP19A1 recombinant protein (Position: Y241-H503). (reported region: Y241-H503).
- Molecular weight context: reported MW: 58 kDa; calculated MW: nan
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
cytochrome P450, family 19, subfamily A, polypeptide 1. CYP19A1, also called Aromatase, is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia. Functional note: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Reported localization: Membrane. Peripheral membrane protein. Expression/tissue context: Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
Research relevance and current trends
- Cancer: Researchers commonly examine how CYP19A1 relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how CYP19A1 relates to this theme using model systems and orthogonal readouts.
- Cytochromes: Researchers commonly examine how CYP19A1 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CYP19A1 levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of CYP19A1 across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
