| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aromatase; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase; CYP19A1; ARO1; CYAR; CYP19 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Aromatase/Cyp19a1 recombinant protein (Position: N75-Q428). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes aromatase (gene Cyp19a1) in human, mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 58.0 kDa, and the supplier reports an observed band at about 49–58 kDa.
Aromatase (CYP19A1) is the cytochrome P450 enzyme that converts androgens into estrogens, turning androstenedione into estrone and testosterone into estradiol. It is expressed in the ovary, placenta, adipose tissue, bone and brain, and it is the target of aromatase inhibitors used in breast cancer, so it is central to studies of estrogen biology and hormone-dependent cancers.
| Target | Aromatase (gene Cyp19a1; UniProt P28649, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 58.0 kDa |
| Observed MW | 49–58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human HepG2 cells, human hcct tissue, human hccp tissue, human U-87MG cells, human MCF-7 cells, human SK-OV-3 cells, human SiHa cells, rat testis tissue, rat ovary tissue, rat brain tissue, rat C6 cells, mouse testis tissue, mouse ovary tissue, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Aromatase/Cyp19a1 fragment (Asn75–Gln428), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aromatase in the endoplasmic reticulum membrane and microsome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Limei et al. (2024) Ti3C2 nanosheet-induced autophagy derails ovarian functions. Journal of Nanobiotechnology. 10.1186/s12951-024-02495-4
Hong Zhao et al. (2005) Electroacupuncture stimulates hypothalamic aromatization. Brain Research. 10.1016/j.brainres.2005.01.004
Zhao Hong et al. (2005) Extragonadal aromatization increases with time after ovariectomy in rats. Reproductive Biology and Endocrinology. 10.1186/1477-7827-3-6
