| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arylsulfatase B; ASB; N-acetylgalactosamine-4-sulfatase; G4S; Arsb; As1; As1-s |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Arsb, which shares 88.2% and 94.1% amino acid (aa) sequence identity with human and rat Arsb, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets arylsulfatase B (gene Arsb) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 59.6 kDa, and the supplier reports an observed band at about 66–70 kDa.
Arylsulfatase B (ARSB, also called N-acetylgalactosamine-4-sulfatase) is a lysosomal enzyme that removes 4-sulfate groups from chondroitin sulfate and dermatan sulfate. Its deficiency causes mucopolysaccharidosis type VI (Maroteaux-Lamy syndrome), and it is also studied in glycosaminoglycan-dependent cell signaling.
| Target | Arylsulfatase B (gene Arsb; UniProt P50429, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 59.6 kDa |
| Observed MW | 66–70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat Rh35 cells, rat C6 cells, mouse HEPA1–6 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of mouse arsb. Sequence identity with the human and rat orthologs: 88.2% and 94.1%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places arylsulfatase B in the lysosome and cell surface. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
