| Field | Specification |
|---|---|
| Target | |
| Alternative names | Set1/Ash2 histone methyltransferase complex subunit ASH2; ASH2-like protein; ASH2L; ASH2L1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ASH2L recombinant protein (Position: N142-P628). Human ASH2L shares 99.2% amino acid (aa) sequence identity with mouse ASH2L. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets set1/Ash2 histone methyltransferase complex subunit ASH2 (gene ASH2L) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
ASH2L is a core subunit of the SET1/MLL (COMPASS-like) histone methyltransferase complexes, together with WDR5, RBBP5 and DPY30. These complexes methylate histone H3 at lysine 4, a mark associated with active gene promoters, and ASH2L is studied in transcriptional regulation and development.
| Target | Set1/Ash2 histone methyltransferase complex subunit ASH2 (gene ASH2L; UniProt Q9UBL3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ASH2L fragment (Asn142–Pro628), expressed in E. coli. Sequence identity with the mouse ortholog: 99.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places set1/Ash2 histone methyltransferase complex subunit ASH2 in the nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
