| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase kinase kinase 5;2.7.11.25;Apoptosis signal-regulating kinase 1;ASK-1;MAPK/ERK kinase kinase 5;MEK kinase 5;MEKK 5;MAP3K5;ASK1, MAPKKK5, MEKK5; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ASK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CFB-13 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase kinase kinase 5 (gene MAP3K5) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 154.5 kDa, and the supplier reports an observed band at about 95 kDa.
ASK1 (apoptosis signal-regulating kinase 1, MAP3K5) is a MAP kinase kinase kinase activated by oxidative stress, ER stress and TNF signaling. It turns on the JNK and p38 pathways through MKK4/7 and MKK3/6 and is held in check by reduced thioredoxin, so it is widely studied in stress-induced apoptosis, inflammation and fibrosis.
| Target | Mitogen-activated protein kinase kinase kinase 5 (gene MAP3K5; UniProt Q99683, human) |
|---|---|
| Clone | CFB-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 154.5 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat stomach tissue, mouse skin tissue, human liver cancer tissue, human placenta tissue, human thyroid papillary carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human ASK1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places mitogen-activated protein kinase kinase kinase 5 in the cytoplasm and endoplasmic reticulum. Tissue expression noted by UniProt (human): Abundantly expressed in heart and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
