| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclic AMP-dependent transcription factor ATF-6 alpha;cAMP-dependent transcription factor ATF-6 alpha;Activating transcription factor 6 alpha;ATF6-alpha;Processed cyclic AMP-dependent transcription factor ATF-6 alpha;ATF6; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ATF6, different from the related mouse and rat sequences by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cyclic AMP-dependent transcription factor ATF-6 alpha (gene ATF6) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 74.6 kDa; the supplier reports observed bands at 90 kDa, 100 kDa.
Cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6) is an ER membrane protein that senses the buildup of misfolded proteins. Under ER stress it moves to the Golgi, where proteases release its cytosolic bZIP domain, which enters the nucleus to activate chaperones and other unfolded protein response genes.
| Target | Cyclic AMP-dependent transcription factor ATF-6 alpha (gene ATF6; UniProt P18850, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 74.6 kDa |
| Observed MW | 90 kDa, 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human ATF6. It differs from the mouse and rat sequences at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclic AMP-dependent transcription factor ATF-6 alpha in the endoplasmic reticulum membrane and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xin Wei et al. (2020) BCAT1 binds the RNA-binding protein ZNF423 to activate autophagy via the IRE1-XBP-1-RIDD axis in hypoxic PASMCs. Cell Death & Disease. 10.1038/s41419-020-02930-y
Daria Fresia et al. (2021) GSH-Independent Induction of ER Stress during Hypoglycaemia in the Retinal Cells of Mice. Journal of Clinical Medicine. 10.3390/jcm10112529
De-Kun Wu et al. (2024) A mechanistic study of Anwei decoction intervention in a rat model of gastric intestinal metaplasia through the endoplasmic reticulum stress–autophagy pathway. Tissue & Cell. 10.1016/j.tice.2024.102317
