| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; ATM |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATM |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BCF-1 is a rabbit monoclonal antibody that recognizes serine-protein kinase ATM in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 350.7 kDa, and the supplier reports an observed band at about 351 kDa.
ATM is a large serine/threonine kinase that is activated by DNA double-strand breaks. It phosphorylates many targets, including p53, CHK2 and histone H2AX, to trigger cell cycle checkpoints and DNA repair, and loss of ATM causes ataxia-telangiectasia.
| Target | Serine-protein kinase ATM (gene ATM; UniProt Q13315, human) |
|---|---|
| Clone | BCF-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 350.7 kDa |
| Observed MW | 351 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human ATM.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine-protein kinase ATM in the nucleus, cytoplasmic vesicle and peroxisome matrix. Tissue expression noted by UniProt (human): Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xu Hui et al. (2022) GBP3 promotes glioblastoma resistance to temozolomide by enhancing DNA damage repair. Oncogene. 10.1038/s41388-022-02397-5
Jun Yan et al. (2026) Identification of a novel chalcone derivative as ferroptosis inducer through targeting TrxR in prostate cancer. Biochemical Pharmacology. 10.1016/j.bcp.2026.117760
