| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/potassium-transporting ATPase subunit alpha-3; Na(+)/K(+) ATPase alpha-3 subunit; Na(+)/K(+) ATPase alpha(III) subunit; Sodium pump subunit alpha-3; ATP1A3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATP1A3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22A98 is a rabbit monoclonal antibody that recognizes sodium/potassium-transporting ATPase subunit alpha-3 (gene ATP1A3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 111.7 kDa, and the supplier reports an observed band at about 112 kDa.
ATP1A3 is the neuron-specific α3 catalytic subunit of the Na+/K+-ATPase. It helps restore ion gradients after intense neuronal firing, and mutations in ATP1A3 cause several neurological disorders, including rapid-onset dystonia-parkinsonism and alternating hemiplegia of childhood.
| Target | Sodium/potassium-transporting ATPase subunit alpha-3 (gene ATP1A3; UniProt P13637, human) |
|---|---|
| Clone | 22A98 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 111.7 kDa |
| Observed MW | 112 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ATP1A3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium/potassium-transporting ATPase subunit alpha-3 in the cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
