| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dual specificity protein phosphatase 1;3.1.3.16;3.1.3.48;Dual specificity protein phosphatase hVH1;Mitogen-activated protein kinase phosphatase 1;MAP kinase phosphatase 1;MKP-1;Protein-tyrosine phosphatase CL100;DUSP1;CL100, MKP1, PTPN10, VH1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human ATP1A3 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ATP1A3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22A98; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-ATP1A3 Rabbit Monoclonal Antibody catalog # M02278. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ATP1A3 (Dual specificity protein phosphatase 1).
- Antibody format: Monoclonal; clone 22A98; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ATP1A3 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle. . Reported cellular localization context: Nucleus . Tissue expression notes (as provided): Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. .
Research relevance and current trends
- Research context keywords from the source record include: Protein Phosphorylation,Ser/Thr Phosphatases,Signal Transduction,Tyrosine Phosphatases.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate ATP1A3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ATP1A3 expression by Western blot in cell or tissue lysates, Compare relative ATP1A3 levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 100-150 kDa; calculated MW: 39298 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 100-150 kDa
- Cellular localization (provided): Nucleus .
- Tissue details (provided): Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
