| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP synthase subunit d, mitochondrial;ATPase subunit d;ATP5H;My032; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ATP synthase peripheral stalk subunit d, mitochondrial (gene ATP5PD) in human, monkey, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 18.5 kDa, and the supplier reports an observed band at about 22 kDa.
ATP5PD is subunit d of the peripheral stalk of mitochondrial ATP synthase (complex V). The peripheral stalk acts as a stator that holds the F1 catalytic head in place while the central rotor turns, enabling ATP synthesis.
| Target | ATP synthase peripheral stalk subunit d, mitochondrial (gene ATP5PD; UniProt O75947, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 18.5 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human U-87MG cells, human A549 cells, monkey COS-7 cells, human SW620 cells, human Jurkat cells, human 293T cells, rat brain tissue, rat liver tissue, rat heart tissue, rat PC-12 cells, mouse brain tissue, mouse liver tissue, mouse heart tissue, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): T-47D cells.
Immunogen
Recombinant human ATP5H fragment (Ala2–Leu161), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 81% and 78%, respectively.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places ATP synthase peripheral stalk subunit d, mitochondrial in the mitochondrion and mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
