| Field | Specification |
|---|---|
| Target | |
| Alternative names | Copper-transporting ATPase 2; Copper pump 2; Wilson disease-associated protein; ATP7B; PWD; WC1; WND |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATP7b |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24A50 is a rabbit monoclonal antibody that recognizes copper-transporting ATPase 2 (gene ATP7B) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 157.3 kDa, and the supplier reports an observed band at about 157 kDa.
ATP7B is a copper-transporting ATPase expressed mainly in the liver. It loads copper onto ceruloplasmin and moves excess copper into bile for excretion, and mutations in ATP7B cause Wilson disease, in which copper accumulates in the liver and brain.
| Target | Copper-transporting ATPase 2 (gene ATP7B; UniProt P35670, human) |
|---|---|
| Clone | 24A50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 157.3 kDa |
| Observed MW | 157 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ATP7b.
Reactivity Notes
The supplier lists reactivity with human. UniProt places copper-transporting ATPase 2 in the late endosome. Tissue expression noted by UniProt (human): Most abundant in liver and kidney and also found in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Yong et al. (2014) A Chinese herbal Formula, Chang-Wei-Qin, Synergistically Enhances Antitumor Effect of Oxaliplatin. Pathology & Oncology Research. 10.1007/s12253-014-9831-5
Da-Long Wu et al. (2006) Expression of ATP7B in human gastric cardiac carcinomas in comparison with distal gastric carcinomas. World Journal of Gastroenterology. 10.3748/wjg.v12.i47.7695
