| Field | Specification |
|---|---|
| Target | |
| Alternative names | BAG family molecular chaperone regulator 5;BAG-5;Bcl-2-associated athanogene 5;BAG5;KIAA0873; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Bag5 recombinant protein (Position: N389-Y447). Human Bag5 shares 96.6% and 93.2% amino acid (aa) sequence identity with mouse and rat Bag5, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets BAG family molecular chaperone regulator 5 (gene BAG5) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 51.2 kDa, and the supplier reports an observed band at about 51 kDa.
BAG5 is a co-chaperone with several BAG domains that acts as a nucleotide exchange factor for HSP70, promoting ADP release and chaperone-assisted protein refolding. It supports protein homeostasis and has been studied in neuronal models, including its interactions with parkin and mitochondrial quality control.
| Target | BAG family molecular chaperone regulator 5 (gene BAG5; UniProt Q9UL15, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 51.2 kDa |
| Observed MW | 51 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human PC-3 cells, human U-87 cells, human HeLa cells, human K562 cells, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): T-47D cells.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human Bag5 fragment (Asn389–Tyr447), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96.6% and 93.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed in the heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
