| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2 homologous antagonist/killer;Apoptosis regulator BAK;Bcl-2-like protein 7;Bcl2-L-7;BAK1;BAK, BCL2L7, CDN1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes bcl-2 homologous antagonist/killer (gene BAK1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 23.4 kDa, and the supplier reports an observed band at about 23 kDa.
BAK (BAK1) is a pro-apoptotic Bcl-2 family effector that resides in the mitochondrial outer membrane. After activation by BH3-only proteins, it oligomerizes to form pores that release cytochrome c and other apoptogenic factors, working alongside BAX in the intrinsic apoptosis pathway.
| Target | Bcl-2 homologous antagonist/killer (gene BAK1; UniProt Q16611, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 23.4 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human THP-1 cells lysate, human MCF-7 cells lysate, human 293T cells lysate, human PC-3 cells lysate, human Hacat cells lysate, human A431 cells lysate, human Caco-2 cells lysate, human RT4 cells lysate, rat thymus tissue lysates, rat heart tissue lysates, rat lung tissue lysates, rat PC-12 cells lysate, mouse thymus tissue lysates, mouse heart tissue lysates, mouse lung tissue lysates, mouse RAW264.7 cells lysate.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 13% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): rat cardiac muscle tissue, human placenta tissue, mouse cardiac muscle tissue.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with confirmed staining (ICC/IF): A549 cells, SMMC-7721 cells.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human BAK fragment (Ala22–Ser211), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places bcl-2 homologous antagonist/killer in the mitochondrion outer membrane. Tissue expression noted by UniProt (human): Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zihao Zhou et al. (2026) Unexpected Promotion of Bone Regeneration by Inhibition of BMPR1A-Mediated BMP Signalling. Cell Proliferation. 10.1111/cpr.70204
Hai-Xia Li et al. (2018) Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study. Oncology Reports. 10.3892/or.2018.6262
