Anti-BAP31 Rabbit Monoclonal Antibody

SKU:BHA21009456
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-BCAP31 antibody (Rabbit monoclonal, clone 19B58, IgG). Validated for Western blot, immunohistochemistry, immunofluorescence and flow cytometry. Reactivity: Human, Mouse. Recognizes B-cell receptor-associated protein 31. Observed band in Western blot: about 25 kDa.
Target BCAP31
clone number 19B58
Host Rabbit
Reactivity Human,Mouse
Isotype IgG
Application(s) WB, IHC, ICC, IF, Flow
Options selector
Catalog no. Size Conjugation
M03767-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC,ICC,IF,Flow Cytometry
      Application details: WB 1:500-2000<br>IHC 1:50-200<br>ICC/IF 1:50-200<br>FC 1:100<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target BCAP31
Alternative names B-cell receptor-associated protein 31; BCR-associated protein 31; Bap31; 6C6-AG tumor-associated antigen; Protein CDM; p28; BCAP31; DXS1357E
UniProt # P51572
Host Rabbit
Clone 19B58
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
Immunogen A synthesized peptide derived from human BAP31
Molecular weight 28.0 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation- induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M03767-1
Main SKU BHA21009456
Rabbit Monoclonal

Product Overview

Clone 19B58 is a rabbit monoclonal antibody that recognizes B-cell receptor-associated protein 31 (gene BCAP31) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 28.0 kDa, and the supplier reports an observed band at about 25 kDa.

BCAP31 (BAP31) is an abundant multi-pass membrane protein of the endoplasmic reticulum that acts as a chaperone and sorting factor. It controls ER export or retention of membrane proteins, helps route misfolded proteins to ER-associated degradation, and when cleaved by caspase-8 it can relay apoptotic signals between the ER and mitochondria.

Target B-cell receptor-associated protein 31 (gene BCAP31; UniProt P51572, human)
Clone 19B58
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 28.0 kDa
Observed MW 25 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunocytochemistry / Immunofluorescence 1:50–1:200
Flow cytometry 1:100

Immunogen

Synthetic peptide based on the sequence of human BAP31.

Reactivity Notes

The supplier lists reactivity with human and mouse. UniProt places B-cell receptor-associated protein 31 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Ubiquitous.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:100. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 28.0 kDa; observed band at about 25 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human BAP31.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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