| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Breast cancer anti-estrogen resistance protein 3;Novel SH2-containing protein 2;SH2 domain-containing protein 3B;BCAR3;NSP2, SH2D3B;UNQ271/PRO308; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human BCAR3, different from the related mouse sequence by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of BCAR3 (Breast cancer anti-estrogen resistance protein 3) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-BCAR3 Antibody Picoband® catalog # PB9846. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human BCAR3, different from the related mouse sequence by five amino acids.
- Molecular weight context: reported MW: 93 kDa; calculated MW: 92566 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Breast cancer anti-estrogen resistance protein 3; Breast cancer anti-estrogen resistance protein 3. Breast cancer anti-estrogen resistance protein 3 is a protein that in humans is encoded by the BCAR3 gene. Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. Functional note: May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. . Reported localization: Secreted. Expression/tissue context: Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue. .
Research relevance and current trends
- Cell Type Markers: Researchers commonly examine how BCAR3 (Breast cancer anti-estrogen resistance protein 3) relates to this theme using model systems and orthogonal readouts.
- Tags & Cell Markers: Researchers commonly examine how BCAR3 (Breast cancer anti-estrogen resistance protein 3) relates to this theme using model systems and orthogonal readouts.
- Tumor Associated: Researchers commonly examine how BCAR3 (Breast cancer anti-estrogen resistance protein 3) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative BCAR3 (Breast cancer anti-estrogen resistance protein 3) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of BCAR3 (Breast cancer anti-estrogen resistance protein 3) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
