| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptosis regulator Bcl-2; BCL2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Bcl-2 recombinant protein (Position: Q118-E165). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects apoptosis regulator Bcl-2 (gene BCL2) in human samples and is validated for ELISA, flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 26.3 kDa, and the supplier reports an observed band at about 26 kDa.
Bcl-2 (BCL2) is an anti-apoptotic protein located mainly on the mitochondrial outer membrane that binds and restrains pro-apoptotic Bcl-2 family members such as BAX, BAK and BH3-only proteins. It was first identified through the t(14;18) translocation in follicular lymphoma, and it is a central regulator of mitochondrial apoptosis and a target of BH3-mimetic drugs.
| Target | Apoptosis regulator Bcl-2 (gene BCL2; UniProt P10415, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 26.3 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | Not specified by the supplier |
Samples with a confirmed band (WB): human Jurkat cells, human HL-60 cells, human THP-1 cells, huamn OE19 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human Bcl-2 fragment (Gln118–Glu165), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places apoptosis regulator Bcl-2 in the mitochondrion outer membrane, nuclear membrane, endoplasmic reticulum membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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