| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | B-cell lymphoma 6 protein;BCL-6;B-cell lymphoma 5 protein;BCL-5;Protein LAZ-3;Zinc finger and BTB domain-containing protein 27;Zinc finger protein 51;BCL6;BCL5, LAZ3, ZBTB27, ZNF51; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Bcl6 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Bcl-6 Rabbit Monoclonal Antibody is an antibody targeting BCL6. Common applications include WB, IHC, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: BAF-2; isotype: Rabbit IgG; reactivity: Human; observed MW: 95 kDa; calculated MW: 78846 MW.
Boster Bio Anti-Bcl-6 Rabbit Monoclonal Antibody catalog # M00142-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: BCL6 — B-cell lymphoma 6 protein
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: BAF-2; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 95 kDa; Calculated: 78846 MW
Biological background
Protein function (datasheet): Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding ly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6- binding site) or inly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, inly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4 (+) T-cells (T (FH) cells), promotes the expression of T (FH)-related genes but inhibits the differentiation of T (H)1, T (H)2 and T (H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53- dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH- dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation. .
Cellular localization (datasheet): Nucleus .
Tissue details (datasheet): Expressed in germinal center T- and B-cells and in primary immature dendritic cells. .
Research relevance and current trends
- Commonly studied in contexts related to Cancer,Cancer Susceptibility,Domain Families,Epigenetics and Nuclear Signaling,Oncoproteins,Oncoproteins/Suppressors,Proto-Oncogenes,Transcription,Transcription Factors,Zinc Finger.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
