| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2-like protein 1;Bcl2-L-1;Apoptosis regulator Bcl-X;BCL2L1;BCL2L, BCLX; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Bcl-X, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes bcl-2-like protein 1 (gene BCL2L1) in human samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 26.0 kDa; the supplier reports observed bands at 29 kDa, 60 kDa.
Bcl-xL (BCL2L1) is an anti-apoptotic Bcl-2 family protein that binds pro-apoptotic partners such as BAX, BAK and BH3-only proteins to prevent mitochondrial outer membrane permeabilization. Alternative splicing produces the pro-apoptotic Bcl-xS isoform. Bcl-xL is important for survival of many cell types, including platelets and neurons, and is a target of BH3-mimetic drugs.
| Target | Bcl-2-like protein 1 (gene BCL2L1; UniProt Q07817, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 26.0 kDa |
| Observed MW | 29 kDa, 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): PC-3 cells, A549 cells.
Immunogen
Synthetic peptide from the middle region of human bcl-X. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xin Guo et al. (2017) Active targeting co-delivery system based on hollow mesoporous silica nanoparticles for antitumor therapy in ovarian cancer stem-like cells. Oncology Reports. 10.3892/or.2017.5829
Qiong Wu et al. (2016) The role of cofilin-l in vulvar squamous cell carcinoma: A marker of carcinogenesis, progression and targeted therapy. Oncology Reports. 10.3892/or.2016.4625
Gongquan Li et al. (2013) Aspirin overcomes Navitoclax-resistance in hepatocellular carcinoma cells through suppression of Mcl-1. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2013.04.018
Liang Zhang et al. (2007) ACTX-8, a cytotoxic l-amino acid oxidase isolated from Agkistrodon acutus snake venom, induces apoptosis in Hela cervical cancer cells. Life Sciences. 10.1016/j.lfs.2006.12.024
