| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2-like protein 1;Bcl2-L-1;Apoptosis regulator Bcl-X;BCL2L1;BCL2L, BCLX; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Bcl-XL |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOD-2 is a rabbit monoclonal antibody that recognizes bcl-2-like protein 1 (gene BCL2L1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 26.0 kDa, and the supplier reports an observed band at about 30 kDa.
Bcl-xL (BCL2L1) is an anti-apoptotic Bcl-2 family protein that binds pro-apoptotic partners such as BAX, BAK and BH3-only proteins to prevent mitochondrial outer membrane permeabilization. Alternative splicing produces the pro-apoptotic Bcl-xS isoform. Bcl-xL is important for survival of many cell types, including platelets and neurons, and is a target of BH3-mimetic drugs.
| Target | Bcl-2-like protein 1 (gene BCL2L1; UniProt Q07817, human) |
|---|---|
| Clone | AOD-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.0 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human 293T cells, human SH-SY5Y cells, rat spleen tissue, rat C6 cells, mouse spleen tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human pituitary tumor tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human bcl-XL.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Han Zhou et al. (2025) CircGNAQ Promotes Intracranial Aneurysm Formation by Facilitating Vascular Smooth Muscle Cell Phenotypic Switching and Apoptosis. Antioxidants & Redox Signaling. 10.1177/15230864251380271
